pHRE1-km vector (V005504)

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LOCUS       40924_24937        9037 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHRE1-km, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Broad-host-range cloning vector pHRE1-km for promoter trapping
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2003) Department of Biochemistry and Molecular 
            Biology, Universidade Federal do Parana-UFPR, Centro Politecnico, 
            Curitiba, Parana 81531-990, Brazil
REFERENCE   3  (bases 1 to 9037)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9037)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-APR-2003) Department of Biochemistry and Molecular Biology, 
            Universidade Federal do Parana-UFPR, Centro Politecnico, Curitiba, 
            Parana 81531-990, Brazil"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     2614..2630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2985..3776)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(3865..3914)
                     /note="promoter region of neomycin and kanamycin resistance
                     aph (NPTII) gene"
                     /regulatory_class="promoter"
     CDS             complement(4709..6517)
                     /label=GUS
                     /note="beta-glucuronidase"
     primer_bind     complement(6555..6571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6613..7326)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(7969..8826)
                     /label=AmpR
                     /note="beta-lactamase"