pHQRep(3X) vector (V005516)

LOCUS       40924_24847        5165 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHQRep(3X), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     An efficient plant transient expression vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2014) School of Life Science, LanZhou University 
            of Technology, Qili River Street, Lanzhou, Gansu 730050, China
REFERENCE   3  (bases 1 to 5165)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2014) School of Life Science, LanZhou University of 
            Technology, Qili River Street, Lanzhou, Gansu 730050, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2404..2458
                     /note="contains three repeated upstream GAL4-DNA bindng
                     sites"
     regulatory      2471..2583
                     /label=partial 35S promoter
                     /note="partial 35S promoter"
                     /regulatory_class="promoter"
     promoter        2526..2572
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     intron          2606..2795
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2790..4613
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             4620..4637
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4672..4924
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4946..4962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4970..4986)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4994..5024)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5039..5060)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."