pHPdestVenus vector (V005522)

Download GeneBank File(.gb)

LOCUS       40924_24817       12196 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning and transformation vector pHPdestVenus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Boy AL, Zhai Z, Habring-Mueller A, Kaspar P, Lohmann I.
  TITLE     Vectors for efficient and high-throughput construction of 
            fluorescent Drosophila reporters using the PhiC31 site-specific 
            integration system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Lohmann I, Boy AL, Habring-Mueller A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2009) CellNetworks - Cluster of Excellence and 
            BIOQUANT Center, University of Heidelberg, Im Neuenheimer Feld 267, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12196)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2009) CellNetworks - Cluster of Excellence and BIOQUANT 
            Center, University of Heidelberg, Im Neuenheimer Feld 267, 
            Heidelberg 69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    63..182
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        212..242
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             296..952
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1297..1599
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1643..1767)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1779..1911
                     /label=Hsp70 promotor
                     /note="Hsp70 promotor"
                     /regulatory_class="promoter"
     CDS             1980..2696
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     CDS             2703..2726
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     polyA_signal    2856..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3201..3270)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    3298..3683
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     gene            3684..7810
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(7821..8406)
                     /label=P element 5' end
     misc_feature    8407..8634
                     /label=white linker sequence
                     /note="white linker sequence"
     rep_origin      complement(8641..9229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9403..10260)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10261..10365)
                     /label=AmpR promoter
     misc_feature    10664..11166
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    complement(11167..11399)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    11798..12183
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"