pHP13 vector (V005524)

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LOCUS       V005524                 4748 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005524
VERSION     V005524
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Upregulated transcription of plasmid and chromosomal ribulose
            monophosphate pathway genes is critical for methanol assimilation
            rate and methanol tolerance in the methylotrophic bacterium Bacillus
            methanolicus
  JOURNAL   J. Bacteriol. 188 (8), 3063-3072 (2006)
   PUBMED   16585766
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Department of Biotechnology, Norwegian
            University of Science and Technology, Sem Saelands vei 6/8,
            Trondheim 7491, Norway
REFERENCE   3  (bases 1 to 4748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2006"; volume: "188"; issue: "8"; pages:
            "3063-3072"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-NOV-2005) Department of Biotechnology, Norwegian University of
            Science and Technology, Sem Saelands vei 6/8, Trondheim 7491,
            Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      565..1153
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1441..1462
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1477..1507
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1515..1531
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1539..1555
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1604..1620)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1832..2479)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(join(4313..4748,1..296))
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"