pHN1_lacUV5 vector (V005529)

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LOCUS       40924_24762        4885 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHN1_lacUV5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Niederholtmeyer H, Wolfstadter BT, Savage DF, Silver PA, Way JC.
  TITLE     Engineering cyanobacteria to synthesize and export hydrophilic 
            products
  JOURNAL   Appl. Environ. Microbiol. 76 (11), 3462-3466 (2010)
  PUBMED    20363793
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Niederholtmeyer H, Wolfstadter BT, Savage DF, Silver PA, Way JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2010) Systems Biology, Harvard Medical School, 200
            Longwood Ave., Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 4885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "11"; 
            pages: "3462-3466"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2010) Systems Biology, Harvard Medical School, 200 Longwood 
            Ave., Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(21..609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1564..1650
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1742..1769
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    complement(1791..1810)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        1825..1855
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    1863..1879
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1888..1902
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1903..1926
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        1934..2036
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2037..2693
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        2761..2838
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2839..3918
                     /label=lacI
                     /note="lac repressor"