pHK vector (V005535)

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LOCUS       V005535                 3523 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005535
VERSION     V005535
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu YJ.
  TITLE     Efficient whole-cell-catalyzing cellulose saccharification using
            engineered Clostridium thermocellum
  JOURNAL   Biotechnol Biofuels 10, 124 (2017)
   PUBMED   28507596
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu Y-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) Metabolomics Group, Qingdao Institute of
            Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,
            189 Songling Rd., Qingdao 266101, P. R. China
REFERENCE   3  (bases 1 to 3523)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 124 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2017) Metabolomics Group, Qingdao Institute of Bioenergy and
            Bioprocess Technology, Chinese Academy of Sciences, 189 Songling
            Rd., Qingdao 266101, P. R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(110..131)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(264..911)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1021..2022)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(2714..3302)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"