pHIV-iRFP720-E2A-Luc vector (V005536)

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LOCUS       V005536                 9053 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005536
VERSION     V005536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Plagge A.
  TITLE     Multimodal cell tracking from implantation to tumour growth by
            combining gold nanorods, iRFP720 and luciferase labelling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   Plagge A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Cellular and Molecular Physiology,
            University of Liverpool, Crown Street, Liverpool L69 3BX, UK
REFERENCE   3  (bases 1 to 9053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-AUG-2017) Cellular and Molecular Physiology, University of
            Liverpool, Crown Street, Liverpool L69 3BX, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   835..1015
                     /rpt_family="HIV-5'LTR"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1686..1919
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2104..2148
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2297..2338
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2446..2563
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2627..3805
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3870..4817
                     /label="iRFP720"
                     /note="near-infrared fluorescent protein with an emission
                     peak at 720 nm, engineered from a bacterial phytochrome
                     (Shcherbakova and Verkhusha, 2013)"
     CDS             4818..4877
                     /label="E2A"
                     /note="2A peptide from equine rhinitis A virus polyprotein"
     CDS             4878..6527
                     /label="luciferase"
                     /note="firefly luciferase"
     primer_bind     complement(6533..6549)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      6722..6743
                     /label="U3PPT"
                     /note="U3PPT"
                     /regulatory_class="other"
     LTR             7059..7239
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7301..7889)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8063..8920)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8921..9025)
                     /label="AmpR promoter"