pHIT88 vector (V005537)

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LOCUS       40924_24678        3073 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIT88 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H.
  TITLE     In vitro analyses of the production and activity of secondary small 
            interfering RNAs in C. elegans
  JOURNAL   EMBO J. 26 (24), 5007-5019 (2007)
  PUBMED    18007599
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Tabara H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2007) Contact:Hiroaki Tabara Kyoto University, 
            Graduate School of Medicine; Yoshida-Konoe, Building E, Room 208, 
            Sakyo, Kyoto 606-8501, Japan Fax :81-75-753-9311
REFERENCE   3  (bases 1 to 3073)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007"; volume: "26"; issue: "24"; pages: "5007-5019"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2007) Contact:Hiroaki Tabara Kyoto University, Graduate 
            School of Medicine"; volume: " Yoshida-Konoe, Building E, Room 208, 
            Sakyo, Kyoto 606-8501, Japan Fax "; pages: "81-75-753-931"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    18..39
                     /label=trans-splicing leader sequence SL1
                     /note="trans-splicing leader sequence SL1"
     misc_feature    38..59
                     /note="multiple cloning sites containing restriction sites
                     (Stu I, Pst I, Xho I and Xba I)"
     misc_feature    59..119
                     /label=synthetic poly(A)61 region
                     /note="synthetic poly(A)61 region"
     misc_feature    115..131
                     /note="restriction enzyme sites (Bsa I and Spe I) for 
                     linearization of the plasmid"
     terminator      146..193
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(393..981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1155..2012)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2013..2117)
                     /label=AmpR promoter
     rep_origin      complement(2451..2906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3051..3067
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"