pHIND2.2 vector (V005557)

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LOCUS       40924_24573        3656 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIND2.2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Development of the pHIND series of plasmids useful for generating 
            nested deletions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-1999) Biology, Brookhaven National Laboratory, 50 
            Bell Ave., Upton, NY 11973, USA
REFERENCE   3  (bases 1 to 3656)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-1999) Biology, Brookhaven National Laboratory, 50 Bell Ave.,
            Upton, NY 11973, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    68..89
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        104..134
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    142..158
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        200..218
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(225..281)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(284..302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(622..1434)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1736..2324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2498..3355)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3356..3460)
                     /label=AmpR promoter
     primer_bind     3557..3573
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3592..3642
                     /note="F1 phage; used for specifically nicking DNA"