pMono_T vector (V004676)

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LOCUS       40924_31245        5010 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMono_T-vector, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Fursova O, Pogorelko G, Zabotina OA.
  TITLE     An efficient method for transient gene expression in monocots 
            applied to modify the Brachypodium distachyon cell wall
  JOURNAL   Ann. Bot. 110 (1), 47-56 (2012)
  PUBMED    22589326
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Pogorelko GV, Fursova OV, Zabotina OA.
  TITLE     Entry T-vector with monocothyledonous plants specific elements 
            (mono_T-Vector)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5010)
  AUTHORS   Pogorelko GV, Fursova OV, Zabotina OA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2011) BBMB, Iowa State University, ISU, Ames, IA 
            50011, USA
REFERENCE   4  (bases 1 to 5010)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Ann. Bot.";
            date: "2012"; volume: "110"; issue: "1"; pages: "47-56"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (14-SEP-2011) BBMB, Iowa State University, ISU, Ames, IA 50011, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    61..162
                     /label=Zea mays ubiquitin signal peptide sequence
                     /note="Zea mays ubiquitin signal peptide sequence"
     CDS             complement(192..1004)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1064..1777
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     terminator      1797..2046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(2135..2153)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2171..2187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2195..2211)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2219..2249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2264..2285)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2573..3161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3335..4192)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4193..4297)
                     /label=AmpR promoter
     rep_origin      complement(4375..4830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4971..4987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4994..5010
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"