Basic Vector Information
- Vector Name:
- pMO79
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8021 bp
- Type:
- Replicative vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A.
pMO79 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMO79 vector Sequence
LOCUS 40924_31190 8021 bp DNA circular SYN 18-DEC-2018 DEFINITION Replicative vector pMO79, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8021) AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A. TITLE Inactivation of [Fe-S] metalloproteins mediates nitric oxide-dependent killing of Burkholderia mallei JOURNAL PLoS ONE 3 (4), E1976 (2008) PUBMED 18398486 REFERENCE 2 (bases 1 to 8021) AUTHORS Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M, Vazquez-Torres A. TITLE Direct Submission JOURNAL Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 80045, USA REFERENCE 3 (bases 1 to 8021) TITLE Direct Submission REFERENCE 4 (bases 1 to 8021) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2008"; volume: "3"; issue: "4"; pages: "E1976" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 80045, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8021 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 96..200 /label=AmpR promoter CDS 201..992 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS 1010..1822 /codon_start=1 /label=KanR /note="aminoglycoside phosphotransferase" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" promoter 1881..1909 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 1924..2637 /codon_start=1 /label=GFPuv /note="GFP variant optimized for excitation by UV light" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" promoter complement(3290..3392) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" misc_feature 3542..4561 /label=mobilization region /note="mobilization region" rep_origin 4785..5554 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 5555..6214 /codon_start=1 /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" protein_bind 6842..6863 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 6878..6908 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6916..6932 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6940..6956 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 7070..7726 /codon_start=1 /label=mRFP1.1 /note="mRFP1.1 is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.." /translation="VQEETMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKG GPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQD SSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKD GGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGA"
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