pMM22 vector (V004700)

LOCUS       40924_31120        4849 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMM22, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Dean C, Barkan D, Bermingham A, Blais J, Casey F, Casarez A, Colvin 
            R, Fuller J, Jones A, Li C, Lopez S, Metzger L, Prathapam R, Rasper 
            D, Reck F, Ruzin A, Shaul J, Shen X, Simmons R, Skewes-Cox P, 
            Tamrakar P, Uehara T.
  TITLE     The monobactam LYS228: mode of action and mechanisms decreasing in 
            vitro susceptibility of Escherichia coli and Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Wei J-R., Mostafavi M, Takeoka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2018) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 4849)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4849)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2018) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(83..1501)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        1738..1766
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1955..2485
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        2794..2822
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     misc_RNA        2828..2847
                     /product="gRNA variable region"
     misc_RNA        2848..2923
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      2945..2979
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      3019..3474
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      3585..4173
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4359..4499)
                     /label=bom
                     /note="basis of mobility region from pBR322"