Basic Vector Information
pML1381 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pML1381 vector Sequence
LOCUS 40924_31085 5540 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pML1381, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5540) AUTHORS DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE. TITLE An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose JOURNAL Nat. Chem. Biol. 11 (7), 465-471 (2015) PUBMED 25984720 REFERENCE 2 (bases 1 to 5540) AUTHORS DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber JE. TITLE Direct Submission JOURNAL Submitted (22-APR-2015) Department of Bioengineering, University of California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 94704, USA REFERENCE 3 (bases 1 to 5540) TITLE Direct Submission REFERENCE 4 (bases 1 to 5540) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-APR-2015) Department of Bioengineering, University of California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 94704, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5540 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 5..524 /label=similar to LEU2 5' /note="similar to LEU2 5'" misc_feature 687..692 /label=BsmBI recognition site /note="BsmBI recognition site" misc_feature 694..697 /label=BsmBI recognition site /note="BsmBI recognition site" misc_feature complement(764..767) /label=BsmBI recognition site /note="BsmBI recognition site" misc_feature complement(769..774) /label=BsmBI recognition site /note="BsmBI recognition site" promoter 1177..1584 /label=LEU2 promoter CDS 1585..2676 /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" regulatory 2680..3156 /regulatory_class="terminator" misc_feature 3161..3670 /label=similar to LEU2 3' /note="similar to LEU2 3'" regulatory complement(3683..3779) /regulatory_class="terminator" CDS complement(3780..4637) /label=AmpR /note="beta-lactamase" promoter complement(4638..4742) /label=AmpR promoter rep_origin 4845..5433 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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