pML1381 vector (V004706)

Basic Vector Information

      • Vector Name:
      • pML1381
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5540 bp
      • Type:
      • Expression vector
      • Source/Author:
      • DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.

pML1381 vector Vector Map

pML13815540 bp60012001800240030003600420048005400similar to LEU2 5'BsmBI recognition siteBsmBI recognition siteBsmBI recognition siteBsmBI recognition siteLEU2 promoterLEU2similar to LEU2 3'AmpRAmpR promoterori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pML1381 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_31085        5540 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pML1381, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in 
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
  PUBMED    25984720
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber 
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA
REFERENCE   3  (bases 1 to 5540)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..524
                     /label=similar to LEU2 5'
                     /note="similar to LEU2 5'"
     misc_feature    687..692
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    694..697
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    complement(764..767)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    complement(769..774)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     promoter        1177..1584
                     /label=LEU2 promoter
     CDS             1585..2676
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      2680..3156
                     /regulatory_class="terminator"
     misc_feature    3161..3670
                     /label=similar to LEU2 3'
                     /note="similar to LEU2 3'"
     regulatory      complement(3683..3779)
                     /regulatory_class="terminator"
     CDS             complement(3780..4637)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4638..4742)
                     /label=AmpR promoter
     rep_origin      4845..5433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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