pMK33-NTAP(GS) vector (V004714)

LOCUS       40924_30990        8793 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMK33-NTAP(GS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Kyriakakis P, Tipping M, Abed L, Veraksa A.
  TITLE     Tandem affinity purification in Drosophila: The advantages of the 
            GS-TAP system
  JOURNAL   Fly (Austin) 2 (4) (2008) In press
  PUBMED    18719405
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Veraksa A, Kyriakakis P, Tipping M, Abed L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2008) Biology Department, University of 
            Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE   3  (bases 1 to 8793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) 2 (4) (2008) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2008) Biology Department, University of Massachusetts 
            Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(865..1453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1627..2484)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2485..2589)
                     /label=AmpR promoter
     promoter        4071..4350
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable
                     element copia (Sinclair et al., 1986)"
     CDS             4396..5418
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6424..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6559..6985
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             7379..7399
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             7406..7426
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             7433..7546
                     /label=SBP
                     /note="streptavidin-binding peptide"
     misc_feature    7547..7576
                     /note="unique cloning sites: XhoI, BamHI, EcoRV, KpnI,
                     SpeI; polylinker"