pMG401 vector (V004746)

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LOCUS       V004746                 4469 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004746
VERSION     V004746
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Quorum sensing resilience against fluid flow
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2015) Health Science and Technology, ETH Zurich,
            Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland
REFERENCE   3  (bases 1 to 4469)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2015) Health Science and Technology, ETH Zurich,
            Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(70..786)
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     RBS             793..804
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     RBS             985..996
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1003..1605
                     /gene="lasI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P33883"
     RBS             1713..1724
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1731..2444
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2535..2621
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2791..3336)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3450..3544)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3578..4369)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"