Basic Vector Information
- Vector Name:
- pMF332
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8294 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Freitag M, Selker EU.
pMF332 vector Map
pMF332 vector Sequence
LOCUS 40924_30740 8294 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pMF332, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8294) AUTHORS Freitag M, Selker EU. TITLE Expression and Visualization of Red Fluorescent Protein (RFP) in Neurospora crassa JOURNAL Fungal Genet. Newsl. 52 (2006) In press REFERENCE 2 (bases 1 to 8294) AUTHORS Freitag M, Selker EU. TITLE Direct Submission JOURNAL Submitted (20-OCT-2005) Institute of Molecular Biology, University of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA REFERENCE 3 (bases 1 to 8294) TITLE Direct Submission REFERENCE 4 (bases 1 to 8294) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Genet. Newsl. 52 (2006) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-OCT-2005) Institute of Molecular Biology, University of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8294 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 363..467 /label=AmpR promoter CDS 468..1325 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1499..2087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2345..2363 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2638..2649 /codon_start=1 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" /translation="IEGR" promoter 4008..4026 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 4056..4072 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 4181..4858 /codon_start=1 /label=dTomato /note="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFL" promoter complement(5614..5632) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(join(8293..8294,1..17)) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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