pMET7hIL-2Ralpha vector (V004760)

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LOCUS       V004760                 3858 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004760
VERSION     V004760
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3858)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..632
                     /label="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human
                     T-lymphotrophic virus 1"
     intron          662..755
                     /label="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
     promoter        800..818
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             861..1676
                     /gene="IL2RA"
                     /label="Interleukin-2 receptor subunit alpha"
                     /note="Interleukin-2 receptor subunit alpha from Homo
                     sapiens. Accession#: P01589"
     polyA_signal    1763..1897
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2107..2695)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2869..3726)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3727..3831)
                     /label="AmpR promoter"