pmET32b vector (V004761)

LOCUS       V004761                 4993 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004761
VERSION     V004761
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
  TITLE     Nucleotide sequence of bacterial ghost creator vector 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi
            Pardis, Azadi Sq., Mashhad, Khorasan Razavi 9177948974, Iran
REFERENCE   3  (bases 1 to 4993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi Pardis, Azadi
            Sq., Mashhad, Khorasan Razavi 9177948974, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2347..2375
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2564..3094
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     rep_origin      complement(3256..3711)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3748..3795)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(3862..3879)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3892..4602)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      4650..4677
                     /label="lambda PR"
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             4691..4699
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4706..4978
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"