pMELalpha vector (V004770)

Download GeneBank File(.gb)

LOCUS       V004770                 7305 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004770
VERSION     V004770
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
   PUBMED   10773444
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2003) Institute for Microbiology, Frankfurt
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 7305)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247
            (1-2), 53-61 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-SEP-2003) Institute for Microbiology, Frankfurt University,
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..659
                     /label="UAS deleted MEL1 promoter"
                     /note="UAS deleted MEL1 promoter"
                     /regulatory_class="promoter"
     CDS             660..2072
                     /gene="MEL1"
                     /label="Alpha-galactosidase 1"
                     /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae.
                     Accession#: P04824"
     promoter        complement(2472..2490)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2511..2527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2535..2551)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2559..2589)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2604..2625)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2913..3501)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3675..4532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4533..4637)
                     /label="AmpR promoter"
     misc_feature    4674..5177
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5436..5656
                     /label="URA3 promoter"
     CDS             5657..6457
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(6589..7044)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7189..7205
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7212..7230
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7263..7279
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"