pMCS vector (V004804)

LOCUS       V004804                12362 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004804
VERSION     V004804
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Xi J, Wang X, Dong L, Dong Y, Su Y, Tang Q, Wang Z.
  TITLE     Construction of a standard reference plasmid containing seven target
            genes for the detection of transgenic cotton
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   Xi J, Wang X, Dong L, Dong Y, Su Y, Tang Q, Wang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2014) Biosafet Lab, Biotechnology Research
            Institute, No. 12 Zhongguancun South Street, Beijing 100081, China
REFERENCE   3  (bases 1 to 12362)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12362)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-APR-2014) Biosafet Lab, Biotechnology Research Institute, No. 12
            Zhongguancun South Street, Beijing 100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4957..5748)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6509..7297)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7366..8043)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8234..8255
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8270..8300
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8308..8324
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8332..8348
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             8364..8633
                     /codon_start=1
                     /gene="cptI"
                     /product="cowpea trypsin inhibitor"
                     /label="cptI"
                     /protein_id="AII71483.1"
                     /translation="MRMDLKHLGSNHHDDSSDEPSESSEPCCDSCICTKSIPPQCHCTD
                     IRWNSCHSACKSCMCTRSMPGKCRCLDIADFCYKPCKSRDEDDE"
     gene            8364..8633
                     /gene="cptI"
                     /label="cptI"
     CDS             complement(8551..9579)
                     /codon_start=1
                     /gene="Cry1Ab/1Ac"
                     /product="Cry1Ab/Cry1Ac fusion protein"
                     /label="Cry1Ab/1Ac"
                     /note="Bt protein"
                     /protein_id="AII71484.1"
                     /translation="NCLSNPEVVVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVL
                     GPVDIIWGIFGPSQWDAFLVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREW
                     EADPTNPALREEMRIQFNDMNSALTTAIPLFAVQNYQVPLLSVYVQAANLHLSVLRDVS
                     VFGQRWGFDAATINSRYNDLTRLIGNYTDHAVRWYNTGLERVWGPDSRDWIRYNQFRRE
                     LTLTVLDIVSLFPNYDSRTYPIRTVSQLTREIYTNPVLENFDGSFRGSAQGIEGSIRSP
                     HLMDILNSITIYTDAHRGEYYWSGHQIMASPVGFIIRRLRHEICTVCSRSPRCPGNGIY
                     PA"
     gene            complement(8551..9579)
                     /gene="Cry1Ab/1Ac"
                     /label="Cry1Ab/1Ac"
     CDS             9595..10959
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     regulatory      10963..11180
                     /label="NOS"
                     /note="NOS"
                     /regulatory_class="terminator"
     CDS             join(11182..11296,11671..12064)
                     /codon_start=1
                     /gene="SadI"
                     /product="AE9 stearoyl-ACP desaturase"
                     /label="SadI"
                     /note="from cotton K312"
                     /protein_id="AII71486.1"
                     /translation="STMPSPRNLRSSLALLFHQRPPLDLPSFP*SPPFLLAPKRLGI*K
                     SLSRLQRRCLFRSPTPCRLTRLRSLNLWRAGLRTTF*LTSNQLRNVGNPPTFFQILILM
                     DFMSKSKSLGKGQRRSQMITL*FWLVI*SPRKPFQLIKQCLIPWMELVMRQVLASLALA
                     VVLQRR"
     gene            11182..12064
                     /gene="SadI"
                     /label="SadI"
     primer_bind     complement(12038..12054)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    12257..12281
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"