pMC212 vector (V004810)

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LOCUS       V004810                13012 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004810
VERSION     V004810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Functional heterologous expression of an engineered full length CipA
            from Clostridium thermocellum in Thermoanaerobacterium
            saccharolyticum
  JOURNAL   Biotechnol Biofuels 6 (1), 32 (2013)
   PUBMED   23448319
REFERENCE   2  (bases 1 to 13012)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 13012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels"; date: "2013"; volume: "6"; issue: "1"; pages: "32"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2013) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1500
                     /label="similar to xynA upstream region"
                     /note="similar to xynA upstream region"
     CDS             1501..3723
                     /gene="celS"
                     /label="Cellulose 1,4-beta-cellobiosidase (reducing end)
                     CelS"
                     /note="Cellulose 1,4-beta-cellobiosidase (reducing end)
                     CelS from Acetivibrio thermocellus (strain ATCC 27405 / DSM
                     1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 /
                     VPI 7372). Accession#: A3DH67"
     CDS             3739..3765
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             3966..4952
                     /codon_start=1
                     /product="pta"
                     /label="pta"
                     /protein_id="AGG53972.1"
                     /translation="MSIIQNIIEKAKSDKKKIVLPEGAEPRTLKAAEIVLKEGIADLVL
                     LGNEDEIRNAAKDLDISKAEIIDPVKSEMFDRYANDFYELRKNKGITLEKARETIKDNI
                     YFGCMMVKEGYADGLVSGAIHATADLLRPAFQIIKTAPGAKIVSSFFIMEVPNCEYGEN
                     GVFLFADCAVNPSPNAEELASIAVQSANTAKNLLGFEPKVAMLSFSTKGSASHELVDKV
                     RKATEIAKELMPDVAIDGELQLDAALVKEVAELKAPGSKVAGCANVLIFPDLQAGNIGY
                     KLVQRLAKANAIGPITQGMGAPVNDLSRGCSYRDIVDVIATTAVQAQ"
     CDS             4970..6181
                     /codon_start=1
                     /product="ack"
                     /label="ack"
                     /protein_id="AGG53973.1"
                     /translation="MKIMKILVINCGSSSLKYQLIESTDGNVLAKGLAERIGINDSMLT
                     HNANGEKIKIKKDMKDHKDAIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGESFTSS
                     VLINDEVLKAITDCIELAPLHNPANIEGIKACQQIMPNVPMVAVFDTAFHQTMPDYAYL
                     YPIPYEYYTKYRIRRYGFHGTSHKYVSNRAAEILNKPIEDLKIITCHLGNGSSIAAVKY
                     GKSIDTSMGFTPLEGLAMGTRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISG
                     ISSDFRDLEDAAFKNGDERAQLALNVFAYRVKKTIGAYAAAMGGVDVIVFTAGVGENGP
                     EIREFILDGLEFLGFSLDKEKNKVRGKETIISTPNSKVSVMVVPTNEEYMIAKDTEKIV
                     KSIK"
     CDS             6840..7631
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7780..9217
                     /label="similar to xynA downstream region"
                     /note="similar to xynA downstream region"
     rep_origin      complement(9556..10101)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        10213..10241
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(10318..11118)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(11119..11339)
                     /label="URA3 promoter"
     misc_feature    11367..11870
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     oriT            complement(12269..12378)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(12799..12826)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(12918..13004)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"