pMC1-EPS-RtGFP vector (V004815)

LOCUS       V004815                 3751 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004815
VERSION     V004815
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Synthetic Vector pMC1-EPS-RtGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3751)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3751)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2013) Biomaterials "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..50)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     regulatory      58..753
                     /label="eps promoter"
                     /note="eps promoter"
                     /regulatory_class="promoter"
     CDS             754..1470
                     /label="EGFP"
                     /note="enhanced GFP"
     rep_origin      1889..2477
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2800..2833
                     /label="lox66"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2926..3047
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3058..3696)
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     RBS             3703..3714
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     protein_bind    complement(3733..3749)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."