pMBL6 vector (V004819)

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LOCUS       40924_29891        4295 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pMBL6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Knight CD, Cove DJ, Cuming AC, Quatrano RS.
  TITLE     Moss Gene Technology
  JOURNAL   (in) Gilmartin,P.M. and Bowler,C. (Eds.); MOLECULAR PLANT BIOLOGY: 
            285-301; Oxford University Press, Oxford, NY, USA (2002)
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Machuka J, Knight CD, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 4295)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Gilmartin,P.M. and Bowler,C. (Eds.)"; volume: " MOLECULAR PLANT 
            BIOLOGY"; pages: " 285-301; Oxford University Press, Oxford, NY, USA
            (2002"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2005) Centre for Plant Sciences, Leeds University, Leeds, 
            West Yorkshire LS2 9JT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    347..523
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     960..976
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1010..1026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1076..1092)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1100..1116)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1124..1154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1169..1190)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2240..3097)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3098..3202)
                     /label=AmpR promoter
     misc_feature    3247..3354
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        3404..3749
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3779..4295,1..275)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"