pMAM vector (V004839)

Basic Vector Information

Vector Name:
pMAM
Antibiotic Resistance:
Ampicillin
Length:
7632 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Kitts PA.

pMAM vector Map

pMAM7632 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500MMTVsmall t intronSV40 NLSSV40 poly(A) signalSV40 promoterXanthine-guanine phosphoribosyltransferasesmall t intronSV40 NLSSV40 poly(A) signalAmpR promoterAmpRoribom

pMAM vector Sequence

LOCUS       V004839                 7632 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004839
VERSION     V004839
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 7632)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
            Meadow Circle, Palo Alto, CA 94303, USA"
            SGRef: number: 3; type: "Journal Article"
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International
            customers, please contact your local distributor.  For technical
            information, call (415) 424- 8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence
            databases, published literature and other sources, together with
            partial sequences obtained by CLONTECH; this vector has not been
            completely sequenced. If you suspect there is an error in this
            sequence, please contact CLONTECH's Technical Service Department at
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..1320
                     /label="MMTV"
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     intron          1562..1627
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1757..1777
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2202..2336
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2349..2678
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2878..3333
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     intron          3813..3878
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4008..4028
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4453..4587
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        5440..5544
                     /label="AmpR promoter"
     CDS             5545..6402
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6576..7164
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(7350..7490)
                     /label="bom"
                     /note="basis of mobility region from pBR322"

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