pmAlbEPintPlauGH vector (V004840)

LOCUS       V004840                 7538 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004840
VERSION     V004840
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M,
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Establishment of a humanized model of liver using NOD/Shi-scid
            IL2Rgnull mice
  JOURNAL   Biochem. Biophys. Res. Commun. 377 (1), 248-252 (2008)
   PUBMED   18840406
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M,
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Expression vector pmAlbEPintPlauGH DNA, complete sequence
  JOURNAL   Published Only in Database (2008)
REFERENCE   3  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Nakamura M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute
            for Experimental Animals, Biomedical Research Department; 3-25-12
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   4  (bases 1 to 7538)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem.
            Biophys. Res. Commun."; date: "2008"; volume: "377"; issue: "1";
            pages: "248-252"
            SGRef: number: 2; type: "Journal Article"; journalName: "Published
            Only in Database (2008)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute for
            Experimental Animals, Biomedical Research Department; 3-25-12
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        678..3013
                     /label="Alb"
                     /note="Mouse albumin promoter"
     intron          3130..3262
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        3307..3325
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3340..4638
                     /gene="Plau"
                     /label="Urokinase-type plasminogen activator"
                     /note="Urokinase-type plasminogen activator from Mus
                     musculus. Accession#: P06869"
     polyA_signal    4694..5316
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        complement(5350..5368)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5389..5405)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5413..5429)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5437..5467)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5482..5503)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5791..6379)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6553..7410)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7411..7515)
                     /label="AmpR promoter"