pMAK705 vector (V004843)

LOCUS       40924_29686        5593 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAK705, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Hamilton CM, Aldea M, Washburn BK, Babitzke P, Kushner SR.
  TITLE     New method for generating deletions and gene replacements in 
            Escherichia coli
  JOURNAL   J. Bacteriol. 171 (9), 4617-4622 (1989)
  PUBMED    2548993
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Maples VF, Kushner SR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2002) Genetics, University of Georgia, Life 
            Sciences Bldg., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5593)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5593)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1989"; volume: "171"; issue: "9"; pages: 
            "4617-4622"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2002) Genetics, University of Georgia, Life Sciences Bldg., 
            Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      487..709
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             757..1704
                     /label=Rep101(Ts)
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     promoter        complement(3191..3295)
                     /label=AmpR promoter
     promoter        3498..3600
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3601..4257
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     primer_bind     5205..5221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5222..5278
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5291..5307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5315..5331)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5339..5369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5384..5405)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    5506..5593
                     /note="derived from vector pBR325 deposited in GenBank
                     Accession Number L08855"