pMA632 vector (V004851)

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LOCUS       40924_29626        4075 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dual pho-lac fusion vector pMA632, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Alexeyev MF, Winkler HH.
  TITLE     Membrane topology of the Rickettsia prowazekii ATP/ADP translocase 
            revealed by novel dual pho-lac reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Alexeyev MF.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-1998) Microbiology and Immunology, University of 
            South Alabama, LMB Building, Mobile, AL 36688-0001, USA
REFERENCE   3  (bases 1 to 4075)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4075)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-1998) Microbiology and Immunology, University of South 
            Alabama, LMB Building, Mobile, AL 36688-0001, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    532..551
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(1909..1925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2070..2089
                     /label=polylinker
                     /note="polylinker"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3090..3947)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3948..4052)
                     /label=AmpR promoter