pLZ2 vector (V004867)

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LOCUS       V004867                 9272 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004867
VERSION     V004867
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Zhu L, Dalbey RE.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Department of Chemistry and Biochemistry,
            The Ohio State University, RM580, W.12th Ave, Columbus, OH 43210,
            USA
REFERENCE   2  (bases 1 to 9272)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Department of Chemistry and Biochemistry, The Ohio
            State University, RM580, W.12th Ave, Columbus, OH 43210, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..72
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      124..171
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(206..2854)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(2953..3018)
                     /label="lambda N peptide"
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et
                     al., 2004)"
     regulatory      complement(3226..3236)
                     /note="pL; induces expression of T7 RNA polymerase"
                     /regulatory_class="promoter"
     regulatory      complement(3247..3252)
                     /label="lambda left promoter"
                     /note="lambda left promoter"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3270..3275)
                     /label="lambda left promoter"
                     /note="lambda left promoter"
                     /regulatory_class="minus_35_signal"
     promoter        3415..3445
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3453..3469
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3490..4200
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     CDS             complement(4656..5468)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5469..5560)
                     /label="AmpR promoter"
     rep_origin      complement(5576..6325)
                     /direction=LEFT
                     /label="RSF ori"
                     /note="Plasmids containing the RSF 1030 origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(6487..6508)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6524..7603)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(7604..7680)
                     /label="lacI promoter"
     promoter        7698..7716
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7781..7802
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7817..7847
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7855..7871
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7879..7895
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(7911..7927)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      8007..8013
                     /regulatory_class="ribosome_binding_site"
     CDS             8021..9271
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"