pLX-Z4-PmasRFP-ALCR vector (V004874)

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LOCUS       V004874                 7308 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004874
VERSION     V004874
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
   PUBMED   28657330
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics,
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid,
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 7308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid
            28049, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      27..58
                     /regulatory_class="terminator"
     misc_feature    59..83
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(106..129)
                     /note="left border of left T-DNA; LB"
     promoter        179..559
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             570..1283
                     /label="TagRFP-T"
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     mat_peptide     1281..1355
                     /product="T2A"
     CDS             1302..1355
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1356..3818
                     /gene="alcR"
                     /label="Regulatory protein alcR"
                     /note="Regulatory protein alcR from Emericella nidulans
                     (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
                     M139). Accession#: P21228"
     terminator      3824..4076
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    4131..4154
                     /label="RB"
                     /note="RB"
     regulatory      complement(4225..4277)
                     /regulatory_class="terminator"
     CDS             complement(4341..4871)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(5060..5088)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    5089..5112
                     /label="assembly linker"
                     /note="assembly linker"
     CDS             complement(5166..6311)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(6704..7235)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"