pLX-R4 vector (V004879)

LOCUS       40924_29416        3865 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-R4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3865)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(308..478)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(527..557)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    653..676
                     /label=RB
                     /note="RB"
     regulatory      complement(741..781)
                     /regulatory_class="terminator"
     misc_feature    791..814
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(872..1402)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1591..1619)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1620..1643
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1697..2842)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(3235..3766)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"