pLV.pA+.GS.Luc vector (V004907)

LOCUS       V004907                 9698 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004907
VERSION     V004907
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Goncalves MA, Janssen JM, Holkers M, de Vries AA.
  TITLE     Rapid and sensitive lentivirus vector-based conditional gene
            expression assay to monitor and quantify cell fusion activity
  JOURNAL   PLoS ONE 5 (6), E10954 (2010)
   PUBMED   20532169
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 9698)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-DEC-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(1943..1990)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(1943..1976)
                     /note="FRT site; Saccharomyces cerevisiae site-specific
                     recombinase FLP recognition target site"
     regulatory      complement(2003..2506)
                     /label="Homo sapiens GAPDH promoter"
                     /note="Homo sapiens GAPDH promoter"
                     /regulatory_class="promoter"
     polyA_signal    complement(2907..2962)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(3129..4778)
                     /label="luciferase"
                     /note="firefly luciferase"
     protein_bind    complement(4837..4884)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(4936..5321)
                     /label="murine metallothionein polyadenylation signal"
                     /note="murine metallothionein polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     CDS             5682..6122
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             6214..6447
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6519..6653
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6680..6815
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6836..6854)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6864..6880)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7025..7480
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7509..7613
                     /label="AmpR promoter"
     misc_feature    7798..8175
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      8644..9232
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9520..9541
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9556..9586
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9594..9610
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9618..9634
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9655..9673
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"