Basic Vector Information
- Vector Name:
- pLV.pA+.GS.Luc
- Length:
- 9698 bp
- Type:
- Lentivirus shuttle vector
- Replication origin:
- ori
- Source/Author:
- Goncalves MA, Janssen JM, Holkers M, de Vries AA.
- Promoter:
- RSV
pLV.pA+.GS.Luc vector Vector Map
pLV.pA+.GS.Luc vector Sequence
LOCUS V004907 9698 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004907
VERSION V004907
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9698)
AUTHORS Goncalves MA, Janssen JM, Holkers M, de Vries AA.
TITLE Rapid and sensitive lentivirus vector-based conditional gene
expression assay to monitor and quantify cell fusion activity
JOURNAL PLoS ONE 5 (6), E10954 (2010)
PUBMED 20532169
REFERENCE 2 (bases 1 to 9698)
AUTHORS Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands
REFERENCE 3 (bases 1 to 9698)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9698)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-DEC-2009) Molecular Cell Biology, Leiden University Medical
Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9698
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1799..1916
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind complement(1943..1990)
/label="FRT"
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
misc_feature complement(1943..1976)
/note="FRT site; Saccharomyces cerevisiae site-specific
recombinase FLP recognition target site"
regulatory complement(2003..2506)
/label="Homo sapiens GAPDH promoter"
/note="Homo sapiens GAPDH promoter"
/regulatory_class="promoter"
polyA_signal complement(2907..2962)
/label="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(3129..4778)
/label="luciferase"
/note="firefly luciferase"
protein_bind complement(4837..4884)
/label="FRT"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
regulatory complement(4936..5321)
/label="murine metallothionein polyadenylation signal"
/note="murine metallothionein polyadenylation signal"
/regulatory_class="polyA_signal_sequence"
CDS 5682..6122
/gene="X"
/label="Protein X"
/note="Protein X from Hepatitis B virus genotype D subtype
ayw (isolate France/Tiollais/1979). Accession#: P03165"
LTR 6214..6447
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6519..6653
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 6680..6815
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(6836..6854)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6864..6880)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 7025..7480
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7509..7613
/label="AmpR promoter"
misc_feature 7798..8175
/label="ISS"
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
rep_origin 8644..9232
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9520..9541
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9556..9586
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 9594..9610
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9618..9634
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 9655..9673
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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