Basic Vector Information
- Vector Name:
- pLV.FLPe.PurR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9650 bp
- Type:
- Lentivirus shuttle vector
- Replication origin:
- ori
- Source/Author:
- Goncalves MA, Janssen JM, Holkers M, de Vries AA.
- Promoter:
- RSV
pLV.FLPe.PurR vector Vector Map
pLV.FLPe.PurR vector Sequence
LOCUS V004913 9650 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004913
VERSION V004913
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9650)
AUTHORS Goncalves MA, Janssen JM, Holkers M, de Vries AA.
TITLE Rapid and sensitive lentivirus vector-based conditional gene
expression assay to monitor and quantify cell fusion activity
JOURNAL PLoS ONE 5 (6), E10954 (2010)
PUBMED 20532169
REFERENCE 2 (bases 1 to 9650)
AUTHORS Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
TITLE Direct Submission
JOURNAL Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands
REFERENCE 3 (bases 1 to 9650)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9650)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-DEC-2009) Molecular Cell Biology, Leiden University Medical
Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9650
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 454..579
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1072..1305
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1489..1533
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1682..1723
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1795..1912
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1935..2238
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2239..2442
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2577..3872
/label="FLPo"
/note="nuclear-targeted site-specific recombinase"
misc_feature 4069..4646
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4674..5270
/label="PuroR"
/note="puromycin N-acetyltransferase"
CDS 5633..6073
/gene="X"
/label="Protein X"
/note="Protein X from Hepatitis B virus genotype D subtype
ayw (isolate France/Tiollais/1979). Accession#: P03165"
LTR 6165..6398
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6470..6604
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 6631..6766
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(6787..6805)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6815..6831)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6976..7431
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7460..7564
/label="AmpR promoter"
CDS 7565..8422
/label="AmpR"
/note="beta-lactamase"
rep_origin 8596..9184
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9472..9493
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9508..9538
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 9546..9562
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9570..9586
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 9607..9625
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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