pLuxR-wt vector (V004917)

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LOCUS       V004917                 5157 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004917
VERSION     V004917
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2005) Electrical Engineering, Princeton
            University, J-319, Engineering Quadrangle, Olden Street, Princeton,
            NJ 08544, USA
REFERENCE   3  (bases 1 to 5157)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5157)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JAN-2005) Electrical Engineering, Princeton University, J-319,
            Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(125..1204)
                     /label="lacI"
                     /note="lac repressor"
     RBS             1208..1216
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1428..2177
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     terminator      2291..2377
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    2502..2523
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2538..2568
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2576..2592
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2600..2616
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     RBS             2681..2692
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             2699..3412
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(3571..4159)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4247..4341)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4365..5021)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5022..5124)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"