pLS975 vector (V004935)

Download GeneBank File(.gb)

LOCUS       V004935                 3451 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004935
VERSION     V004935
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma C, Shang GD.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2009) College of Life Sciences, Nanjing Normal
            University, No. 1 Wenyuan Rd., XiXia District, Nanjing, Jiangsu
            210046, P.R. China
REFERENCE   2  (bases 1 to 3451)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3451)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-OCT-2009) College of Life Sciences, Nanjing Normal University,
            No. 1 Wenyuan Rd., XiXia District, Nanjing, Jiangsu 210046, P.R.
            China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     806..822
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(844..860)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             878..1246
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     promoter        complement(1263..1281)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1302..1318)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1326..1342)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1350..1380)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1395..1416)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2466..3323)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3324..3428)
                     /label="AmpR promoter"