pLR6-Ppy-luc(+) vector (V004943)

Download GeneBank File(.gb)

LOCUS       V004943                 6743 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004943
VERSION     V004943
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutants of
            beetle luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2016) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie gory, Bld. 1/11, Moscow, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 6743)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2016) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie gory, Bld. 1/11, Moscow, Moscow 119991,
            Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx forward primer binding region"
                     /note="F_start_pLRx forward primer binding region"
     CDS             1615..3249
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             3259..3276
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3343..3390
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3427..3882
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3909..4013
                     /label="AmpR promoter"
     CDS             4014..4871
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5045..5633
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5819..5959)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6064..6252)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"