pLR6-Eluc vector (V004945)

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LOCUS       V004945                 6731 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004945
VERSION     V004945
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutants of
            beetle luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6731)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx forward primer binding region"
                     /note="F_start_pLRx forward primer binding region"
     RBS             3087..3095
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3247..3264
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3331..3378
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3415..3870
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3897..4001
                     /label="AmpR promoter"
     CDS             4002..4859
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5033..5621
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5807..5947)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6052..6240)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"