pLR4a vector (V004948)

LOCUS       V004948                 6749 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004948
VERSION     V004948
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in E. coli cells to screen and compare mutants of beetle
            luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx"
                     /note="F_start_pLRx"
     CDS             1615..3252
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     CDS             3265..3282
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3349..3396
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3433..3888
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3915..4019
                     /label="AmpR promoter"
     CDS             4020..4877
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5051..5639
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5825..5965)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6070..6258)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"