pLR3 vector (V004950)

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LOCUS       V004950                 6270 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004950
VERSION     V004950
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant
            enzymes with bioluminescence spectra showing low pH sensitivity
  JOURNAL   Biochemistry Mosc. 73 (8), 862-869 (2008)
   PUBMED   18774932
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 6270)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Biochemistry Mosc."; date: "2008"; volume: "73"; issue: "8"; pages:
            "862-869"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2010) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             1615..3258
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     promoter        3436..3540
                     /label="AmpR promoter"
     CDS             3541..4398
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4572..5160
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5346..5486)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(5591..5779)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"