Basic Vector Information
- Vector Name:
- pLOLA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4156 bp
- Type:
- Plant expression vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Ferrando A, Koncz-Kalman Z, Farras R, Tiburcio A, Schell J, Koncz C.
- Promoter:
- CaMV 35S
pLOLA vector Map
pLOLA vector Sequence
LOCUS 40924_28581 4156 bp DNA circular SYN 18-DEC-2018 DEFINITION Plant expression vector pLOLA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4156) AUTHORS Ferrando A, Koncz-Kalman Z, Farras R, Tiburcio A, Schell J, Koncz C. TITLE Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells JOURNAL Nucleic Acids Res. 29 (17), 3685-3693 (2001) PUBMED 11522840 REFERENCE 2 (bases 1 to 4156) AUTHORS Berendzen KW, Koncz C. TITLE Direct Submission JOURNAL Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany REFERENCE 3 (bases 1 to 4156) TITLE Direct Submission REFERENCE 4 (bases 1 to 4156) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "2001"; volume: "29"; issue: "17"; pages: "3685-3693" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4156 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..18 /note="Tth111I, NotI, XhoI, KpnI; MCS promoter" promoter 409..754 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 759..793 /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream" misc_feature 794..808 /label=N-term c-myc epitope /note="N-term c-myc epitope" intron 809..997 /label=IV2 intron /note="modified second intron of the potato ST-LS1 gene (Vancanneyt et al., 1990)" misc_feature 998..1012 /label=C-term c-myc epitope /note="C-term c-myc epitope" misc_feature 1013..1040 /note="SalI, BamHI, PstI, EcoRI; MCS downstream" regulatory 1041..1293 /note="polyadenylation signal from nopaline synthase gene of the Agrobacterium Ti plasmid; NOS terminator" /regulatory_class="terminator" promoter complement(1325..1343) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1373..1389) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1397..1413) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1421..1451) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1466..1487) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1775..2363) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2537..3394) /label=AmpR /note="beta-lactamase" promoter complement(3395..3499) /label=AmpR promoter rep_origin 3526..3981 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 4122..4138 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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