pLN-ENR-GFP vector (V004968)

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LOCUS       40924_28521        8416 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLN-ENR-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2007) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   5  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-JAN-2007) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2009) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Jul 10, 2009 this sequence version replaced DQ813653.2.
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(40..876)
                     /label=derived from hsp86 3' UTR
                     /note="derived from hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(885..1595)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    complement(1602..2897)
                     /gene="enr-gfp"
                     /label=ENR
                     /note="ENR"
     misc_feature    complement(2905..3916)
                     /label=contains promoter and 5' UTR from calmodulin gene
                     /note="contains promoter and 5' UTR from calmodulin gene"
     misc_feature    3999..4593
                     /label=contains promoter and 5' UTR from PcDT gene
                     /note="contains promoter and 5' UTR from PcDT gene"
     CDS             4611..5003
                     /label=BSD
                     /note="blasticidin S deaminase"
     regulatory      5012..5608
                     /label=derived from hrp2 3' UTR
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    complement(5622..5728)
                     /label=tandem attP elements
                     /note="tandem attP elements"
     misc_recomb     complement(5622..5672)
                     /label=attP
                     /note="attP"
     misc_recomb     complement(5679..5728)
                     /label=attP
                     /note="attP"
     promoter        complement(5734..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6248..6352
                     /label=AmpR promoter
     CDS             6353..7210
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7384..7972
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8260..8281
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8296..8326
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8334..8350
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8358..8374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8392..8410
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"