pLMB51 vector (V004973)

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LOCUS       V004973                13893 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004973
VERSION     V004973
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS.
  TITLE     Regulatable vectors for environmental gene expression in
            alphaproteobacteria
  JOURNAL   Appl. Environ. Microbiol. 78 (19), 7137-7140 (2012)
   PUBMED   22820336
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   Poole PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2012) Molecular Microbiology, John Innes Centre,
            Colney Lane, Norwich NR47UH, UK
REFERENCE   3  (bases 1 to 13893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "19";
            pages: "7137-7140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-APR-2012) Molecular Microbiology, John Innes Centre, Colney
            Lane, Norwich NR47UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(733..1392)
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label="parA"
                     /protein_id="AFR24674.1"
                     /translation="MATREQQPEGRRLKVARIYLRASTDEQNLERQESLVAATRAAGYY
                     VAGIYREKASGARADRPELLRMIADLQPGEVVVAEKIDRISRLPLAEAERLVASIRAKG
                     AKLAVPGVVDLSELAAEANGVAKIVLESVQDMLLKLALQMARDDYEDRRERQRQGVQLA
                     KAAGRYTGRKRDAGMHDRIITLRSGGSSIAKTAKLVGCSPSQVKRVWAAWNAQQQK"
     gene            complement(733..1392)
                     /gene="parA"
                     /label="parA"
     CDS             complement(1356..1886)
                     /gene="parB"
                     /label="Protein ParB"
                     /note="Protein ParB from Escherichia coli. Accession#:
                     P22997"
     CDS             complement(1883..2176)
                     /codon_start=1
                     /gene="parC"
                     /product="ParC"
                     /label="parC"
                     /note="partition protein"
                     /protein_id="AFR24675.1"
                     /translation="MGIPNLTKGDAMRAAKHYRQLLSIDFNIEALAFVPGPDGTRGRRI
                     HVLGREVRDRPGLVEYLSPAFGSRVALDGYCKANFDAVLHLAYPDHQQWGHA"
     gene            complement(1883..2176)
                     /gene="parC"
                     /label="parC"
     CDS             2327..2575
                     /gene="parD"
                     /label="Antitoxin ParD"
                     /note="Antitoxin ParD from Escherichia coli. Accession#:
                     P22995"
     CDS             2575..2883
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     primer_bind     complement(3289..3305)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3313..3329)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3337..3367)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3382..3403)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            complement(3594..3702)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        3885..3989
                     /label="AmpR promoter"
     CDS             3990..4847
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(5373..6569)
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             6675..7322
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     rep_origin      7814..8517
                     /label="oriV"
                     /note="incP origin of replication"
     CDS             9184..10329
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10383..12191)
                     /label="GUS"
                     /note="beta-glucuronidase"
     regulatory      12241..12397
                     /gene="tauAp_tauR"
                     /regulatory_class="promoter"
     gene            12241..12397
                     /gene="tauAp_tauR"
                     /label="tauAp_tauR"