pLM53 vector (V004975)

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LOCUS       40924_28476        7232 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM53, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Novel Escherichia coli strains to detect protein-protein 
            interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7232)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-1999) Biochemistry and Biophysics, Texas A "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2650..2838
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2943..3083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3269..3857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4031..4888)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4889..4980)
                     /label=AmpR promoter
     terminator      complement(4999..5026)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5118..5204)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(5503..6159)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(6231..6285)
                     /gene="cat"
                     /label=pL promoter
                     /note="pL promoter"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="promoter"
     misc_binding    complement(6240..6256)
                     /gene="cat"
                     /label=O-L1 repressor binding site
                     /bound_moiety="O-L1 repressor"
     misc_binding    complement(6264..6280)
                     /gene="cat"
                     /label=O-L2 repressor binding site
                     /bound_moiety="O-L2 repressor"
     promoter        6369..6397
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6586..7116
                     /label=GmR
                     /note="gentamycin acetyltransferase"