pLM3 vector (V004976)

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LOCUS       40924_28471        5042 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Lambda repressor fusion vectors to map protein-protein interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5042)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-1999) Biochemistry and Biophysics, Texas A "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..47
                     /regulatory_class="promoter"
     CDS             73..462
                     /codon_start=1
                     /product="lambda repressor protein"
                     /label=lambda repressor protein
                     /note="DNA binding domain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="AAF01197.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAER"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2103..2291
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2396..2536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2722..3310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3421..3934
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(3979..4836)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4837..4941)
                     /label=AmpR promoter