pLM10 vector (V004977)

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LOCUS       40924_28466        7144 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Marino-Ramirez L, Blum BJ, Hu JC.
  TITLE     Green fluorescent protein (GFP) as a reporter to map protein-protein
            interactions in Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Marino-Ramirez L, Blum BJ, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7144)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7144)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-1998) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2650..2838
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2943..3083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3269..3857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4031..4888)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4889..4980)
                     /label=AmpR promoter
     terminator      complement(4999..5026)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5118..5204)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(5503..6159)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6217..6247)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     regulatory      6276..6330
                     /gene="gfp"
                     /allele="mut2"
                     /label=pL promoter
                     /note="pL promoter"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="promoter"
     misc_binding    6281..6295
                     /gene="gfp"
                     /allele="mut2"
                     /label=O-L2 repressor binding site
                     /bound_moiety="O-L2 repressor"
     misc_binding    6305..6321
                     /gene="gfp"
                     /allele="mut2"
                     /label=O-L1 repressor binding site
                     /bound_moiety="O-L1 repressor"
     CDS             6376..7089
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      7109..7132
                     /gene="gfp"
                     /allele="mut2"
                     /regulatory_class="attenuator"