pLM005-RBCS1-Venus vector (V004985)

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LOCUS       40924_28426        7825 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLM005-RBCS1-Venus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Freeman Rosenzweig ES, Xu B, Kuhn Cuellar L, Marinez-Sanchez A, 
            Schaffer M, Strauss M, Cartwright HN, Forster F, Engel BD, Wingreen 
            NS, Mackinder LCM., Jonikas MC.
  TITLE     The pyrenoid is liquid-like and exhibits dynamic reorganization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Mackinder LCM., Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2017) Department of Biology, University of York, 
            Wentworth Way, York, North Yorkshire YO10 5DD, United Kingdom
REFERENCE   3  (bases 1 to 7825)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2017) Department of Biology, University of York, Wentworth 
            Way, York, North Yorkshire YO10 5DD, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1256..1972
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELIK"
     CDS             2024..2047
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     rep_origin      complement(3408..3996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4170..5027)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5028..5132)
                     /label=AmpR promoter
     CDS             6011..6814
                     /codon_start=1
                     /product="APHVIII"
                     /label=APHVIII
                     /note="Paromomycin resistance marker"
                     /protein_id="AUR45269.1"
                     /translation="MDDALRALRGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAA
                     LGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRL
                     DVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGER
                     LLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLR
                     ELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEFF"
     promoter        complement(7139..7157)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"