pLM vector (V004987)

LOCUS       40924_28416        6550 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pLM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Mei L.
  TITLE     Construction of high-efficiency baculovirus expression vector and 
            its expression in chicken cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Mei L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2009) Microbiology, Life Science, Xuefu Road, 
            Harbin, Heilongjiang 150080, China
REFERENCE   3  (bases 1 to 6550)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2009) Microbiology, Life Science, Xuefu Road, Harbin, 
            Heilongjiang 150080, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(8..129)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(236..824)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    complement(828..873)
                     /label=MCS
                     /note="MCS"
     promoter        complement(895..1098)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1099..1402)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1569..1660
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     1757..1816
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             1991..2023
                     /codon_start=1
                     /label=VSV-G tag
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
                     /translation="YTDIEMNRLGK"
     polyA_signal    2154..2288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2317..2482)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2666..3121
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3148..3252
                     /label=AmpR promoter
     CDS             3253..4110
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4284..4872
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5175..5399)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5469..5999)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6188..6216)
                     /label=Pc promoter
                     /note="class 1 integron promoter"