pLL1179 vector (V004991)

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LOCUS       V004991                 6917 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004991
VERSION     V004991
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Lo J, Olson DG, Murphy SJ, Tian L, Hon S, Lanahan A, Guss AM, Lynd
            LR.
  TITLE     Engineering electron metabolism to increase ethanol production in
            Clostridium thermocellum
  JOURNAL   Metab. Eng. (2016) In press
   PUBMED   27989806
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Lo J, Olson DG, Murphy SJL., Tian L, Hon S, Lanahan AA, Guss AM,
            Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 6917)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6917)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-NOV-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /regulatory_class="promoter"
     CDS             210..1055
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label="nfnA"
                     /protein_id="APQ46211.1"
                     /translation="MFKIVKKKILNPQVKLMEISAPHVAKKAEPGQFIILRVNENGERI
                     PLTIADFDREKGTVTIIFQEVGKTTKLLGTLEEGDELLDFVGPLGKASHFENVKKAAVI
                     GGGLGTAIAYPQAKKLHSMGVEVHTIAGFRNKDLIILEDEMRAVSSKLFITTDDGSNGN
                     KGFVSDVLKKLIEEGNKYDLVVAIGPLIMMKVISELTRPYGIKTIVSMNPVMIDGTGMC
                     GGCRVTVGGEIKFACVDGPDFDGHLVDFDEAMRRQAMYKKEESLALEKHNCKLGVEKNA
                     "
     gene            210..1055
                     /gene="nfnA"
                     /label="nfnA"
     CDS             1048..2442
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label="nfnB"
                     /protein_id="APQ46210.1"
                     /translation="MPNMSPKKVPMPEQDPNVRIKNFLEVALGYTEQMAMEEAQRCLNC
                     KHKPCVSGCPVNVKIPEFVQLIAQGKFEKAYNKIRETNNLPAICGRVCPQENQCEKFCV
                     RGIKGEPVAIGRLERFAADWHMKNGTTSYEKPEKNGKRVAVIGSGPASLTCASDLAKLG
                     YEVTIFEAFHVPGGVLMYGIPEFRLPKKLVQEEIETIKQLGVEIKTNMVIGKVYSIDEL
                     KAEGYDAIFIGSGAGLPSFMKIPGENLNGVYSANEFLTRINLMKAYEFPNCDTPVKVGK
                     NVAVVGGGNVAMDAARSAKRLGAENVYIVYRRSEAEMPARLEEIHHAKEEGILFKFLTN
                     PTRILGTDDGWVKGMECIEMELGEPDESGRRRPVPKPGSEHVIDVETVIIAIGQTPNPL
                     IASTTPGLATQKWGGIIVDENTGATNIEGVYAGGDAVTGAATVILAMGAGKKAAKAIDE
                     YLKNKK"
     gene            1048..2442
                     /gene="nfnB"
                     /label="nfnB"
     CDS             2572..3219
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3303..4160)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4161..4252)
                     /label="AmpR promoter"
     rep_origin      4852..5397
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5687..5867
     CDS             5868..6869
                     /label="repB"
                     /note="RepB replication protein"