Basic Vector Information
- Vector Name:
- pLH5000
- Antibiotic Resistance:
- Streptomycin
- Length:
- 8958 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Hausmann L, Toepfer R.
- Promoter:
- CaMV 35S
pLH5000 vector Map
pLH5000 vector Sequence
LOCUS 40924_28237 8958 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pLH5000, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8958)
AUTHORS Hausmann L, Toepfer R.
TITLE Development of Plasmid Vectors
JOURNAL (in) Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.); BIOENGINEERING
OF CUSTOM-TAILORED RAPE VARIETIES: 155-172; GPZ e. V., Von
Sieboldstr. 8, Goettingen, Germany (1999)
REFERENCE 2 (bases 1 to 8958)
AUTHORS Hausmann L, Toepfer R.
TITLE Direct Submission
JOURNAL Submitted (12-FEB-2003) Institute for Grapevine Breeding
Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE 3 (bases 1 to 8958)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8958)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "(in)
Brauer,D., Roebbelen,G. and Toepfer,R. (Eds.)"; volume: "
BIOENGINEERING OF CUSTOM-TAILORED RAPE VARIETIES"; pages: " 155-172;
GPZ e. V., Von Sieboldstr. 8, Goettingen, Germany (1999"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-FEB-2003) Institute for Grapevine Breeding Geilweilerhof,
Siebeldingen 76833, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8958
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 80..424
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 446..1006
/label=DHFR
/note="mouse dihydrofolate reductase"
regulatory 1060..1259
/note="terminator sequence from the 35S gene of Cauliflower
mosaic virus in GenBank Accession Numbers X05868 and
V00140"
/regulatory_class="terminator"
misc_difference 1098
/label=compared to GenBank Accession Number X05868
/note="compared to GenBank Accession Number X05868"
/experiment="experimental evidence, no additional details
recorded"
regulatory 1225..1240
/regulatory_class="polyA_signal_sequence"
misc_feature 1255
/label=putative transcription stop site
/note="putative transcription stop site"
misc_feature 1286..1387
/note="multiple cloning site from vector pBlueSfi BA in
GenBank Accession Number AF327875"
regulatory 1293..1302
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_difference 1413
/replace="a"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_feature 1432..1456
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
repeat_region 1480..1492
/label=repeat C'
/note="repeat C'"
misc_difference 1486
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
repeat_region 1515..1526
/label=repeat C
/note="repeat C"
repeat_region 1540..1550
/label=repeat B
/note="repeat B"
repeat_region 1567..1577
/label=repeat B
/note="repeat B"
misc_difference 1572
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_difference 1583
/replace="a"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
misc_feature complement(1600..1990)
/note="3' sequence of the ornithine cyclodeaminase gene
ocd; non-functional"
misc_difference 1776
/replace="t"
/label=compared to GenBank Accession Number X07435
/note="compared to GenBank Accession Number X07435"
/experiment="experimental evidence, no additional details
recorded"
rep_origin 2101..2689
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_difference 2787..2788
/replace="gta"
/label=compared to GenBank Accession Number J01749
/note="compared to GenBank Accession Number J01749"
/experiment="experimental evidence, no additional details
recorded"
misc_feature complement(2874..3014)
/label=bom
/note="basis of mobility region from pBR322"
regulatory 3302..3307
/regulatory_class="minus_35_signal"
regulatory 3328..3333
/regulatory_class="minus_10_signal"
regulatory 3350..3354
/regulatory_class="ribosome_binding_site"
CDS 3364..4050
/codon_start=1
/product="resolvase"
/label=resolvase
/note="resolvase-like protein encoded by parR of plasmid
pVS1; similar to Tn3 resolvase in GenBank Accession Number
V00613, Tn917 resolvase in GenBank Accession Number M11180,
and RK2 ParA in GenBank Accession Number L27758"
/protein_id="AAO85346.1"
/translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
RQEEQA"
misc_difference 3783
/replace="c"
/note="original nucleotide changed to destroy a SfiI
restriction site"
/experiment="experimental evidence, no additional details
recorded"
stem_loop 4194..4226
/label=palindromic sequence
/note="palindromic sequence"
misc_difference 4202
/replace="g"
/note="nucleotide changed to conserve palindromic sequence
following SfiI restriction site-destruction"
/experiment="experimental evidence, no additional details
recorded"
misc_difference 4217
/replace="c"
/note="original nucleotide changed to destroy two SfiI
restriction sites"
/experiment="experimental evidence, no additional details
recorded"
regulatory 4284..4289
/regulatory_class="minus_35_signal"
regulatory 4305..4310
/regulatory_class="minus_10_signal"
regulatory 4338..4345
/regulatory_class="ribosome_binding_site"
CDS 4349..4975
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
misc_feature 5245..5257
/note="identical to the korB operator binding site of
plasmid RK2 in GenBank Accession Number L27758.1"
CDS 5407..6477
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
stem_loop 6494..6516
/label=palindromic sequence
/note="palindromic sequence"
rep_origin 6546..6740
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
repeat_region 6837..6850
/label=repeat E
/rpt_unit_range=6837 .. 6843
/note="repeat E"
regulatory 7081..7086
/regulatory_class="minus_35_signal"
regulatory 7104..7109
/regulatory_class="minus_10_signal"
CDS 7345..8133
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
stem_loop 8139..8196
/note="palindromic sequence; putative termination signal"
misc_difference 8373..8374
/replace="ggtacc"
/note="compared to GenBank Accession Number X00493; removed
to destroy a KpnI restriction site"
misc_feature 8656..8680
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
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