pLeo697 vector (V005021)

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LOCUS       V005021                 7683 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005021
VERSION     V005021
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
   PUBMED   29686358
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 7683)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..215
                     /label="Psv40"
                     /note="Psv40"
     promoter        18..214
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      65..200
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     regulatory      240..249
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             273..3002
                     /gene="IL6ST"
                     /label="Interleukin-6 receptor subunit beta"
                     /note="Interleukin-6 receptor subunit beta from Homo
                     sapiens. Accession#: P40189"
     polyA_signal    3056..3280
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3326..3754
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3768..4097
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4164..4955
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5132..5265
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5302..5318)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5326..5342)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5350..5380)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5395..5416)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5704..6289)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6463..7320)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7321..7425)
                     /label="AmpR promoter"