pLeo696 vector (V005022)

LOCUS       V005022                 6354 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005022
VERSION     V005022
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
   PUBMED   29686358
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 6354)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..215
                     /label="Psv40"
                     /note="Psv40"
     promoter        18..214
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      65..200
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     sig_peptide     246..308
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             312..1673
                     /gene="IL6R"
                     /label="Interleukin-6 receptor subunit alpha"
                     /note="Interleukin-6 receptor subunit alpha from Homo
                     sapiens. Accession#: P08887"
     polyA_signal    1727..1951
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1997..2425
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2439..2768
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2835..3626
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3803..3936
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3973..3989)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3997..4013)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4021..4051)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4066..4087)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4375..4960)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5134..5991)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5992..6096)
                     /label="AmpR promoter"